With a fast scan speed and multiplexing capabilities, the q exactive plus mass spectrometer is an outstanding detector for fast chromatography separation techniques. This guide is intended for all personnel who operate the q exactive mass spectrometer. Metworks software is compatible with all thermo scienti. Isq lt, tsq 8000 evo, and q exactive gc mass spectrometers. Based on an innovative combination of a hexabore capillary and ionfunnel based entrance optics which allow maximum capture of your analytes, this is the ideal qtof lcms for your proteomics research. The results illustrate the benefits of the integration of ce and esi in a single dynamic process coupled with high resolution mass spectrometry. Mass spectrometrybased proteomics using q exactive, a high. Exactive plus operating manual planet orbitrap mafiadoc. You can access pdf files of the documents listed above from the data system computer. Calibrating thermo exactive with the direct analysis in real. If you have a q exactive gc system, go to start all programs thermo instruments manuals.
Log in your mass spec qexactive session on the cores. This q exactive plus quickstart guide gives an introduction on setting up and using the thermo scientific q exactive plus mass spectrometer. Jun 03, 2011 the q exactive also employs a 90 bent ion path from the source toward the mass analyzer in common with the exactive and in contrast to the ltq orbitrap instruments. Demonstrates how to remove and clean the slens on the q exactive instrument series removing and cleaning the slens on thermo scientific q exactive mass spectrometers on vimeo join. Q exactive gc operating manual pn 1r1207060002, revision a i. Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer. The image current from the detector is processed by an enhanced fourier transformation algorithm, doubling mass spectrometric resolution. Thermo scientific q exactive hf orbitrap lcmsms system. Send us an email if you are looking for a specific resource or advice on how you can get the most out of your instrument. Thermo scientific orbitrap fusion series hardware manual. Thermo scientific q exactive plus orbitrap liquid chromatographyhigh resolution mass spectrometry lchrms system. It is specific to the thermo scientific q exactive mass spectrometer and will include instruction for electrospray ionization esi of proteins and peptides. The superior quality of q exactive plus msms data enables identification and quantitation of more compounds with greater confidence. Sciex, coupled to a thermo qexactive mass spectrometer was used for the analyses.
Thermo scientific q exactive plus orbitrap lcmsms system. Thermo scientific q exactive orbitrap lcmsms system. The tsq 8000 evo system provides the selectivity and sensitivity of a triplequadrupole gcms while also functioning as a highperformance single quadrupole. The peakheight level is usually specified as a percentage of the maximum peak height. Thus, it is mandatory to read also the q exactive operating manual and the ion max and ion maxs api source hardware manual. Manuals for the delivered ion sources and other software. Thermo fisher scientific exactive operating manual vii identifying safety information the exactive operating manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. The computer is not recognizing the mass spec thermo scientific q exactive. A more detailed and thorough account of these procedures can be found in the qexactive operating manual. Warning symbols alert the user to check for hazardous conditions. For information about performing these tasks, see the exactive operating manual, the exactive software manual, or the exactive tune online help, and the ion max and ion maxs api source hardware manual. It is capable of ionization using conventional heated electrospray hesi, atmospheric pressure chemical ionization apci, and nanoflow electrospray nanoesi. Exactive operating manual manuals for the delivered ion sources and other software. Mass spectrometers department of chemistry mellon college of.
The user will need to create a user defined mass calibration list in order to calibration the exactive using the custom calibration solution outlined in section 1. Thermo scientific q exactive orbitrap lcmsms system from. This q exactive instrument features high ion currents because of an slens, and fast highenergy collisioninduced dissociation peptide fragmentation because of parallel filling and detection modes. Q exactive hf software manual thermo fisher scientific. Thermo scientific content manual and documents official.
Exactive quickstart guide thermo fisher scientific. Thermo scientific exactive series operating manual pn bre0012255, revision a vii technical data for exactive series systems the table summarizes the most import ant technical data of the exactive series systems. Exactive plus operating manual pn 23060, revision a i. To view product manuals go to start programs thermo exactive manuals. Removing and cleaning the slens on thermo scientific q. The q exactive is an excellent system that generates reproducible, solid datasets. Upgrade your maps peptide mapping thermo scientific. Thermo q exactive the exactive plus orbitrap lcms is a benchtop full scan mass spectrometer designed for compound identification and highthroughput, highperformance screening. Q exactive hybrid quadrupole orbitrap mass spectrometer. All other trademarks are the property of thermo fisher scientific inc. The thermo scientific q exactive gc hybrid quadrupole orbitrap mass spectrometer provides an unmatched combination of sensitivity, massaccuracy and resolving power, helping answer todays most challenging analytical questions. Learn more about the 6550 ifunnel qtof lcms, the most sensitive lcqtof in the agilent portfolio.
Pulsed q collision induced dissociation pqd enables trapping of low mass. About this manual this exactive plus operating manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. This course is designed for users that have previous lcms experience and are interested in protein and peptide analysis. Tune file on the desktop, or go to start all programs thermo exactive series tune.
What you need to know about calibration and maintenance of the thermo scientificq exactive mass spectrometer series. We know you, or someone you know, worked very hard to get your orbitrapbased mass spectrometer in your lab and even harder preparing your precious samples. Recent publications have reported methods using 19and 24variable windows implemented on q exactive and q exactive hf at a resolution of 30,000 7,8, and 70 windows on q exactive hfx with fixed. This guide is intended for all personnel who operate the q exactive plus mass spectrometer. It is simple to use and maintenance on the q exactive is. Please note that this guide does not contain any safe ty notices and precautionary statements. The thermo qexactive orbitrap is a high resolution mass spectrometer suitable for accurate mass measurement as well as msms studies.
Mass spectrometrybased proteomics using q exactive, a. Operating manual for instructions about using the lifting devices. Utilization of high resolution lcms for screening and quantitative analysis of pesticides in food matrix using a q exactive bench top orbitrap platform. With bestinclass performance characteristics for both quantitative and discovery workflows, the q exactive.
Q exactive hybrid quadrupoleorbitrap ms with zipchip system a comprehensive ceesims solution for biopharma applications. There are several ways to contact thermo fisher scientific. We use it in the lab to perform shotgun proteomic and metaproteomic analysis of biological specimens within various studies. Resources for optimal performance of your orbitrap fusion series mass spectrometer. Scope of this guide the q exactive hf software manual includes the following chapters. In order to generate a user defined mass calibration list, the current user role setting in the exactives instrument status window must be selected as advanced. In addition to this manual, thermo fisher scientific provides other documents for the exactive plus mass spectrometer that. It is an easytouse, dedicated benchtop gcms system that provides the highest confidence in compound discovery, identification and quantitation for a comprehensive understanding of your samples. This q exactive hf software manual is intended for all personnel that need to perform measurements with the q exactive hf mass spectrometer, especially the key operator. An instrument with attached lifting devices does not fit through a door. Thermo scientific q exactive mass spectrometer series calibration and maintenance.
Q exactive resolution at mz 200 free injection time ms approx. Exactive gc orbitrap gcms and q exactive gc orbitrap gcms. The q exactive hfx ms takes identification and quantitation. Thermo scientific user manuals download manualslib. Thermo scientific tsq 8000 evo user guide ix p preface this guide contains detailed information about how to use your thermo scientific tsq 8000 evo triplequadrupole gcms system.
In addition, metworks software allows highresolution, accuratemass data from orbitrap, ltq ft, q exactive and exactive instruments to be fully leveraged. Q exactive gc orbitrap gcmsms thermo fisher scientific. Scope of this guide the exactive software manual includes the following chapters. Thermo fisher scientific is pleased to announce the release of thermo scientific watson lims 7. Thermo orbitrap fusion series hardware manual pdf download.
Faster, more powerful speed productivity confidence the thermo scientific q exactive hf hybrid quadrupoleorbitrap mass spectrometer features an ultrahighfield orbitrap analyzer which doubles its speed and resolution more samples run and amazing data quality. Thanks to its orbitrap mass analyzer, the exactive plus system delivers highresolution, accuratemass hram fullscan ms for fast, precise and. Tsq 8000 evo, and q exactive gc direct probe system user guide, pn 1r1205050006 to view product manuals if you have an isq lt or a tsq 8000 evo system, open the manuals folder on your desktop. Exactive plus orbitrap mass spectrometer thermo scientific. Isq 7000 user guide isq 7000 hardware manual isq 7000 spare parts guide isq series, tsq series and q exactive gc direct probe user guide tsq 9000 triple quadrupole ms 5 tsq 9000 preinstallation requirements guide tsq 9000 user guide tsq 9000 hardware manual tsq 9000 spare parts guide isq series, tsq series and q exactive gc direct probe user guide. Tmt workflow on the q exactive series instrument parameter optimization and data analysis in proteome discoverer 2. The thermo q exactive orbitrap is a high resolution mass spectrometer suitable for accurate mass measurement as well as msms studies. The data system computer with the q exactive hf tune software is switched on and the mass spectrometer is placed in standby mode. It is capable of ionization using conventional heated electrospray hesi, atmospheric pressure chemical ionization. See the respective chapters of the manual for details and. The thermo scientific tm q exactive tm hybrid quadrupoleorbitrap mass spectrometer introduced the revolutionary concept of quanfirmation tm.
This exactive software manual is intended for all personnel that need to perform measurements with the exactive mass spectrometer, especially the key operator. Recent publications have reported methods using 19and 24variable windows implemented on qexactive and qexactive hf at a resolution of 30,000 7,8, and 70 windows on q. The q exactive hf system combines a stateoftheart segmented quadrupole for highperformance precursor ion selection with a highresolution, accuratemass hram ultrahighfield orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. Today, the q exactive gc system makes this comprehensive analysis a reality. This text is a guide to operating the qexactive mass spectroscopy. Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive. Rapid level 3 characterization of biologics using a cesi 8000. See the respective chapters of the manual for details and additional instrument properties. The defining difference of the q exactive compared with the exactive is the presence of a mass selective quadrupole analyzer between the ion source and the ctrap fig. It is simple to use and maintenance on the q exactive is usually straightforward when outlined in the manual. Resources for optimal performance of your orbitrap elite mass spectrometer.
The thermo scientific exactive plus orbitrap ms is a benchtop lcms system designed for highperformance, highthroughput screening, compound identification, and quantitative analysis. The stateoftheart peptide mapping data possible with the thermo scientific q exactive biopharma platform is a culmination of decades of leadership in the development of data dependent acquisition dda combined with the unmatched accuracy and resolution achieved by the thermo scientific orbitrap mass analyzer. Thermo scientific q exactive hf orbitrap lcmsms system higherquality data, faster than ever the thermo scientific q exactive hf benchtop lcmsms combines a highresolution accuratemass hram ultrahighfield orbitrap analyzer and a high performance quadrupole precursor ion selection to deliver unsurpassed speed while. Thermo scientific exactive series operating manual pn bre0012255, revision a vii technical data for exactive series systems the table summarizes the most import ant technical data of the exactive. To download user documentation from the thermo scientific. Peptides, both large and small 3 65 amino acids, were identified, separated, and quantified.
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